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2 edition of Lactate and the in vitro effect of insulin in frog muscle. found in the catalog.

Lactate and the in vitro effect of insulin in frog muscle.

Desmond Robert Hugh Gourley

Lactate and the in vitro effect of insulin in frog muscle.

by Desmond Robert Hugh Gourley

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Published .
Written in English


Edition Notes

Thesis (PhD) - University of Toronto, 1949.

The Physical Object
Pagination1 v.
ID Numbers
Open LibraryOL20578195M

For example, ranatuerin-2CBd from the American bullfrog L. catesbeianus produced a significant effect on the rate of insulin release at 30 nM [5] and brevinin-2GUb from the Asian frog H. güntheri. Insulin resistance can be defined as an impaired effect of a certain amount of insulin in target tissues, i.e. mainly muscle, fat and liver. Insulin resistance can manifest itself as either unresponsiveness or insensitivity to insulin. Unresponsiveness implies that there is an impaired maximal effect of insulin.

Insulin resistance underlies all forms of diabetes, and is a condition which triglycerides, resulting in insulin resistance. Since muscle tissue occupies more than 40% of the human body by Polimeno GC, et al. Acute effect of fatty acids on metabolism and.   Insulin’s excitatory actions are effectively abolished in the genetically engineered muscle insulin receptor knock‐out (MIRKO) mouse because they have a complete lack of the insulin‐sensitive glucose transport protein, Glut 4, in muscle, so insulin has no stimulatory effect on glucose uptake.

There was no effect of exercise on insulin binding, basal and insulin-stimulated receptor autophosphorylation, or basal and insulin-stimulated exogenous kinase activity, in . Glucagon and insulin regulate in vitro hepatic glycogenolysis in the axolotl Ambystoma mexicanum via changes in tissue cyclic AMP concentration. Gen Comp Endocrinol. ; 61(1) (ISSN: ) Janssens PA; Maher F. Glucagon increases the rate of glycogenolysis in in vitro cultures of hepatic tissue from the axolotl Ambystoma mexicanum.


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Lactate and the in vitro effect of insulin in frog muscle by Desmond Robert Hugh Gourley Download PDF EPUB FB2

The elevation of plasma lactate had rapid effects to suppress insulin-stimulated glycolysis, which clearly preceded its effect to decrease insulin-stimulated glucose uptake. Both submaximal and maximal insulin-stimulated glucose transport decreased % (P Cited by:   Insulin inhibition or protein degradation was decreased with either lactate as sole fuel.

We suggest that the inhibition of protein degradation occurs over the normal range of plasma concentrations of insulin present in vivo and that the presence of glucose may be at least in part necessary for this effect of insulin. The effect of insulin on intact muscle from normal and alloxan-diabetic rats.

J Biol Chem. Jan; –8. MILLER ON, OLSON RE. Metabolism of cardiac muscle. Utilization of pyruvate and DL-Lactate by duck heart. J Biol Chem. Nov; (1)– MORGAN HE, CADENAS E, REGEN DM, PARK CR.

Regulation of glucose uptake in by: Insulin promoted MDA production, glucose consumption and lactate production through ROS production in hepatocellular carcinoma cells. (a) HepG2 cells were seeded in a well plate at 8 × 10 4 cells/well and cultured at 37°C for 24 h.

The cells were then incubated in serum-free medium for 24 h, followed by the pretreatment with catalase ( U/mL) for 1 by: The aim of the present study was to examine the effects of insulin on glucose uptake and glycogen synthesis in crab Chasmagnathus granulata gills.

We observed an increased glucose uptake and incorporation of d-[14 C]glucose into glycogen when posterior C. granulata gills were incubated in the presence of insulin; however, this was not observed in anterior gills, despite the presence of similar.

Thus, insulin has a direct effect on carbohydrate metabolism of frog liver in vitro that is similar to the effect of this hormone on isolated liver tissue from mammals. s experiments with frogs and toads have demonstrated that pancreatectomy elicits a pronounced rise in the concentration of blood glucose (Frye, ; Gorbman, ; Rangnekar.

Insulin facilitates entry of glucose into muscle, adipose and several other tissues. The only mechanism by which cells can take up glucose is by facilitated diffusion through a family of hexose many tissues - muscle being a prime example - the major transporter used for uptake of glucose (called GLUT4) is made available in the plasma membrane through the action of insulin.

THE EFFECTS OF ADMINISTRATION OF GLUCOSE AND INSULIN ON BLOOD PYRUVATE AND LACTATE IN DIABETES MELLITUS BY DANIEL KLEIN (Prom the Metabolic Laboratory and the Division of Metabolic Diseases, Philadelphia General Hospital, Philadelphia) (Received for publication, J.

Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance.

Previous studies suggest that insulin can inhibit hepatic glucose production by both direct and indirect actions. The indirect effects include inhibition of glucagon secretion, reduction in plasma nonesterified fatty acid levels, reduction of the amount of gluconeogenic precursor supplied to the liver, and change in neural input to the liver.

There is a controversy concerning the fact that the. effects of insulin on hepatic glucose production for the treatment of diabetes will be discussed.

Diabetes 55 (Suppl. 2):S65–S69, F or a long time, it was believed that the inhibition of hepatic glucose production (HGP) by insulin resulted only from a direct effect of. T1 - Synchronous fluctuations of blood insulin and lactate concentrations in humans.

AU - Feneberg, Reinhard. AU - Sparber, Monika. AU - Veldhuis, Johannes D. AU - Mehls, Otto. AU - Ritz, Eberhard. AU - Schaefer, Franz. PY - /1/ Y1 - /1/ N2 - Oscillatory organization is a universal mode of signal transduction in living organisms.

The goal of this study was to determine the effect of insulin on the uptake and metabolic fate of lactate and glucose at rest in skeletal muscle of the American bullfrog, Lithobates catesbeiana.

Insulin Stimulated Lactate Production by Jordan Griffin It has been shown that Type 2 diabetic patients who undergo Roux-en-Y Gastric Bypass surgery (RYGB) show a decrease in muscle lactate production, liver glucose production and pancreatic insulin secretion. To explain these changes, it is hypothesized.

Lactate is actively oxidized in red skeletal muscle. In view of glucose uptake and oxidation, in working muscle, lactate oxidation to pyruvate is unlikely to occur in the cytosol, where the revers occurs.

Rather, in skeletal muscle lactate is oxidized in mitochondria (19,–). Krebs, Graves, and Fischer proposed some years ago that Ca*"** ions might be involved in the activation of phosphorylase b kinase, since they observed an activation in vitro (12, 1 3).

4 In the following experiments an attempt was made to study the role of Ca*** in the interconversion of phosphorylase b ^ a in the intact frog sartorius muscle. It has been shown that Type 2 diabetic patients who undergo Roux-en-Y Gastric Bypass surgery (RYGB) show a decrease in muscle lactate production, liver glucose production and pancreatic insulin secretion.

To explain these changes, it is hypothesized that gastric bypass surgery “removes” a block at pyruvate oxidation in both muscle and liver. The effect of insulin on glucose uptake, transfer, and metabolism wasinvestigated in' the human placenta perfused in vitro. Insulin concentrations in maternal perfusion medium were varied from 0– μU/ml, whereas the glucose concentration was kept constant in maternal and fetal perfusion media.

PREGNANCY— Skeletal muscle is the principal site of whole-body glucose disposal, and along with adipose tissue, becomes severely insulin resistant during the latter half of pregnancy. Normal preg-nancy is characterized by an 50% de-crease in insulin-mediated glucose disposal in humans and a –% in-crease in insulin secretion to.

Effect of Insulin on the Proliferation of Cultured Primate Arterial Smooth Muscle Cells By Robert W. Stout, Edwin L. Bierman, and Russell Ross ABSTRACT Smooth muscle cells were grown from thoracic aortas of 1-year-old monkeys (Macaca nemistrina).

The effect of. Insulin is a hormone made by the pancreas that allows your body to use sugar (glucose) from carbohydrates in the food that you eat for energy or to store glucose for future use.

Insulin helps keeps your blood sugar level from getting too high (hyperglycemia) or too low (hypoglycemia).The purpose of this study was to examine the effect of insulin on the utilization of lactate in glucose synthesis in sheep.

The euglycemic model was used in sheep. [U- 14 C]Lactate and [6- 3 H]glucose were infused to monitor lactate and glucose fluxes.Introduction. Insulin is a hormone released by pancreatic beta cells in response to elevated levels of nutrients in the blood.

Insulin triggers the uptake of glucose, fatty acids and amino acids into liver, adipose tissue and muscle and promotes the storage of these nutrients in the form of glycogen, lipids and protein respectively.